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Image Search Results
Journal:
Article Title: Cloning of an Avian Adeno-Associated Virus (AAAV) and Generation of Recombinant AAAV Particles
doi: 10.1128/JVI.77.12.6799-6810.2003
Figure Lengend Snippet: Southern blot analysis of AAAV nuclease-resistant particles in 293T and LMH cells. (A) 293T cells were transfected with pAAAV alone (lane 3), pAAAV plus pAd12 (lane 2), and pAAAV plus infection with wt Ad (lane 1). (B) LMH cells were transfected with pAAAV alone (lane 2) or pAAAV plus infection with FAV1 (lane 1). Viral DNA was isolated as described in Materials and Methods and fractionated on agarose gel before Southern blot analysis with a 32P-labeled pAAAV DNA.
Article Snippet: AAAV (ATCC VR-865) was propagated in 10-day-old Spafas pathogen-free embryonated chicken eggs coinfected with the Phelps strain of
Techniques: Southern Blot, Transfection, Infection, Isolation, Agarose Gel Electrophoresis, Labeling